Estimaing osmolarity by change in volume

Abstract In this experiment we are trying to find at what osmolarity of sucrose solution is isotonic with a potato cylinder. If diffusion will not occur between the potato and sucrose solution at the same osmolarity then there will be a low percentage change in weight and volume between initial and final volume of the potato cylinder.

Estimaing osmolarity by change in volume

Biology Lab - Estimating Osmolarity by Change in Volume of Potato Tuber Tissue - Lab Report

Abstract In this experiment we are trying to find at what osmolarity of sucrose solution is isotonic with a potato cylinder. If diffusion will not occur between the potato and sucrose solution at the same osmolarity then there will be a low percentage change in weight and volume between initial and final volume of the potato cylinder.

We used calipers to measure the diameter and length of each potato cylinder pre and post incubation in their given sucrose solution. The cylinders were made by using a cork borer and were undamaged and at least five cm in length.

Cylinders were incubated for an hour stirring each beaker every 15 minutes. We found that the inside of a potato was isotonic with a 0.

Introduction Scientist always need to determine proper water content for normal processes to happen in plants. Tonicity is defined as the ability of a solution to cause a cell to lose or gain water Freeman et al, There are three classes of tonicity: Isotonic, hypertonic, and hypotonic Sperelakis, A hypertonic solution, the solution has a higher amount of solutes and water tends to come out of the cell or object by diffusion Sperelakis, A hypotonic solution is the exact opposite with a higher solute concentration on the inside of a cell or object than the outside solution so the object tends to swell Sperelakis, In this experiment we will find the osmolarity of a potato cylinder by measuring its change in volume.

Materials and Methods We started by obtaining mL of distilled water and mL of each sucrose solutions and labeled each beaker appropriately. Using a cork borer we obtained seven cylinders of potato making sure they were at least 5cm in length and undamaged.

We then cut the potato cylinders to a uniform length of 5cm and then placed them into a covered Petri dish. Using the caliper we measured the length and diameter of a cylinder to the nearest 0.

We then repeated these steps for the other six potato cylinders placing each one in the appropriate incubating solution 0. Allow the potato cylinders to incubate for 1 hr, making sure to swirl each beaker every 15 minutes.

At the end of the hour record the time each cylinder is removed from its solution and do so in chronological order. Blot each cylinder dry and use the caliper to measure the length and diameter to the nearest 0. When finished be sure to record the data.

Results The potato cylinders were 5cm and length and all of them were undamaged and similar in size. Initial volume for these potato's from smallest osmolarity to the highest were Final volume from least osmolarity to highest osmolarity in sucrose solution was Graph 2 shows the relationship between final and initial volumes at varying osmolarities of sucrose solutions graph 2.

It appears that the least change in volume in a potato was seen at a sucrose osmolarity of 0. Initial and final lengths and diameters of the seven potato cylinders can be found in table and can be compared in graph 1 table 1 graph1.

Sucrose Osmolarity and its Effect on Potato Volume.View Lab Report - Osmolarity Lab from BIOL at University of San Francisco. October 1, Estimating Osmolality by Change in Volume Results: All of the potato tuber cylinders decreased in size%(1).

Osmolarity is affected by changes in water content, as well as temperature and pressure. In contrast, osmolality is independent of temperature and pressure. For a given solution, osmolarity is slightly less than osmolality, because the total solvent weight (the divisor used for osmolality) excludes the weight of any solutes, whereas the total solution volume (used for osmolarity) includes solute content.

Estimating Osmolarity by change in weight. No description The osmolarity of the potato merely depends on the sucrose solution it is placed in. Information Osmolarity: Is a measure of the concentration of all osmotically-active solute particles in a solution.

Estimating Osmolarity of Sweet Potato Presented by: Emely Garcia, Jennifer. A change of 10% to 15% in blood volume and arterial pressure is required to produce the same response. ADH The kidney controls water excretion largely through ADH - a polypeptide secreted by the supraoptic and paraventricular hypothalamic cells with axons ending in the posterior pituitary gland.

The osmolarity of the potato merely depends on the sucrose solution it is placed in.

Estimaing osmolarity by change in volume

Background Information Osmolarity: Is a measure of the concentration of all osmotically-active solute particles in a solution. Estimating Osmolarity of Sweet Potato Presented by: Emely Garcia, Jennifer Price, Alexis Cardenas, & Darius Thornton. Estimaing Osmolarity by Change in Volume.

between the volume of a gas and the temperature of a gas at constant pressure. Verify Charles’s law. BACKGROUND INFORMATION The volume of a gas at constant pressure increases when the temperature of the gas is raised.

This observation was first made by Jacques A. C. Charles in

Estimaing Osmolarity by Change in Volume | Essay Example